MONMOUTH JUNCTION, N.J. - Oct. 10, 2020 - Biotech Support Group reports on a book chapter describing the simplicity and efficiency of their lipid clearance sample preparation technology for the preparation and storage of monoclonal antibodies. The citation is:
Collecting and Storing Hybridoma Tissue Culture Supernatants
doi:10.1101/pdb.prot103317 (http://cshprotocols.cshlp.org/content/2020/10/pdb.prot103317.full)
Cold Spring Harb Protoc 2020.
© 2020 Cold Spring Harbor Laboratory Press (http://cshprotocols.cshlp.org/site/misc/terms.xhtml)
For most immunochemical methods, tissue culture supernatants will be the most useful source of monoclonal antibodies. The supernatants are not contaminated with high levels of other antibodies, and the concentration is high enough for most assays if used undiluted. Procedures for collecting tissue culture supernatants are described. In the Troubleshooting section, the chapter states: "Problem (Step 7): A precipitate of lipids and/or cryoproteins has formed.
Solution: This may be produced by long-term storage at 4°C. These precipitates can be removed …using Cleanascite (Biotech Support Group; X2555) for clarification."
"With the cost and complexity of producing pure monoclonal antibodies, it is essential to have methods that maintain high yields. Here is another citation for the use of Cleanascite™ for the purpose of removing lipids and maintaining high recovery of monoclonal antibodies." states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.
For more information visit: Cleanascite™ Lipid Removal Reagent and Clarification, at
http://www.biotechsupportgroup.com/Cleanascite-Lipid-Removal-Reagent-p/x2555.htm
About Biotech Support Group LLC
Converging with cultural and technological disruptions forthcoming in healthcare, Biotech Support Group develops methods for cost effective and efficient sample prep essential for these expanding markets. Following a tiered business strategy, the company continues its growth in the consumable research products area supporting the rapidly expanding installation of LC-MS instrument and computational infrastructure. For this market, key products include: AlbuVoid™ and AlbuSorb™ for albumin depletion, Cleanascite™ for lipid adsorption, HemogloBind™ and HemoVoid™ for hemoglobin removal, and NuGel™ for functional proteomics. From these innovations, the company has acquired knowledgebase and biomarker intellectual property assets that support discoveries of protein markers from blood, with special emphasis on early detection and personalized medical decisions for cancer patients. For more information, go to http://www.biotechsupportgroup.com/.
For Business Development, contact: Matthew Kuruc 732-274-2866, mkuruc@biotechsupportgroup.com (mailto:sales@biotechsupportgroup.com)
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